人妻有码中文字幕-国产视频一区二区-99热在线观看-在厨房拨开内裤进入毛片

技術文章您現在的位置:首頁 > 技術文章 > 恒遠產品文獻:多聚甲醛試劑引用文獻

恒遠產品文獻:多聚甲醛試劑引用文獻

更新時間:2021-01-11   點擊次數:905次
【文獻標題】Human umbilical cord mesenchymal stem cell-derived exosomes suppress dermal fibroblasts-myofibroblats transition via inhibiting the TGF-β1/Smad 2/3 signaling pathway
【作者】Jian Hu, Yuanwen Chen, Yubin Huang,et.al
【作者單位】深圳市寶安區人民醫院(The People’s Hospital of Bao’an Shenzhen)
【文獻中引用產品】
多聚甲醛
【關鍵詞】Human umbilical cord mesenchymal stem cells,Exosome, TGF-β1/Smad2/3 signaling pathway,Dermal ibroblasts Myofibroblast
【DOI】/doi.org/10.1016/j.yexmp.2020.104468
【影響因子(IF)】2.39
出版期刊】《Experimental and Molecular Pathology》
【產品原文引用】
The hUC-MSCs or fibroblasts in exponential phase were seeded at 1 × 105 cells/mL and 3 mL/well in a 6-well plate with a cover glass. After culture for 24 h at 37℃ with 5% CO2, culture medium was discarded, and cells were fixed with paraformaldehyde (Shanghai HengYuan 
Biological Technology Co., Ltd., Shanghai, China) for 10 min. After discarding the fixative solution, the cover glasses were rinsed by PBS 3 times, each for 3 min. PBS was then absorbed with absorbent papers, normal goat serum was dripped on the glass, and fibroblasts were sealed for 30min at room temperature. The sealing liquid was absorbed by absorbent papers without washes. After that, diluted primary antibodies (supplementary Table 1) were dripped into each cover glass, which was incubated in a wet box at 4℃ overnight. Afterwards, the fluorescent secondary antibody goat anti-rat IgG H&L (Alexa Fluor® 488) (1/200, ab150113) was added. Then the glass was dripped with 4',6-diamidino-2-phenylindole (DAPI) for nucleus staining, and sealed with sealing liquid containing anti-fluorescent quenching agent, and then observed under the fluorescence microscope. The above antibodies were all purchased from Abcam Inc. (Cambridge, MA, USA).
完整版PDF文獻請咨詢在線客服或者聯系我司業務員!
更多公司福利請關注“恒遠生物”VX公眾號!

 




主站蜘蛛池模板: 孕妇特级毛片ww无码内射| 欧美va天堂在线电影| 国产精品久久久久久无码五月| 亚洲欧美日韩中文无线码| 女女女女bbbbbb毛片在线| 风韵少妇性饥渴推油按摩视频| 久久国产avjust麻豆| 国产精品网站在线观看免费传媒| 亚洲av无码乱码在线观看裸奔| 亚洲国产精品无码中文lv| 精品国内自产拍在线观看 | 免费网站看v片在线18禁无码| 97久久国产亚洲精品超碰热| 男女性杂交内射妇女bbwxz | 欧洲av无码放荡人妇网站| 国产女人的高潮国语对白| 久久婷婷五月综合97色| 激情久久av一区av二区av三区| 色综合色国产热无码一| 欧洲熟妇乱xxxxx大屁股7| 无码不卡中文字幕av| jizzjizz亚洲日本少妇| 色久悠悠婷婷综合在线亚洲| 娇妻被打开双腿灌满白浆一区| 波多野结衣绝顶大高潮| 国模吧无码一区二区三区| 爆乳美女午夜福利视频| 亚洲最大成人网色| 在线中文字幕亚洲日韩2020| 51妺嘿嘿午夜福利| 中国凸偷窥xxxx自由视频妇科| 人妻体内射精一区二区三区| 亚洲精品夜夜夜妓女网| 国产免费av片无码永久免费| 黑人巨大精品欧美一区二区| 亚洲欧洲无卡二区视頻| 色欲悠久久久久综合区| 亚洲色自偷自拍另类小说| 国产三级精品三级| 久激情内射婷内射蜜桃| а√最新版在线天堂|