人妻有码中文字幕-国产视频一区二区-99热在线观看-在厨房拨开内裤进入毛片

技術(shù)文章您現(xiàn)在的位置:首頁 > 技術(shù)文章 > 恒遠(yuǎn)產(chǎn)品文獻(xiàn):生物素標(biāo)記的山羊抗兔抗體引用文獻(xiàn)

恒遠(yuǎn)產(chǎn)品文獻(xiàn):生物素標(biāo)記的山羊抗兔抗體引用文獻(xiàn)

更新時(shí)間:2021-01-11   點(diǎn)擊次數(shù):857次

【文獻(xiàn)標(biāo)題】Circular RNA-hsa-circ-0000670 promotes gastric cancer progression through the microRNA-384/SIX4 axis

【作者】Pengliang Liu, Shuang Cai, Nuo Li

【作者單位】中國醫(yī)科大學(xué)附屬第四醫(yī)院(the Fourth Affiliated Hospital of China Medical University)

【文獻(xiàn)中引用產(chǎn)品】

生物素標(biāo)記的山羊抗兔抗體

【關(guān)鍵詞】Hsa-circ-0000670,MicroRNA-384,Sine oculis-related homeobox 4,Gastric cancer

【DOI】doi.org/10.1016/j.yexcr.2020.112141

【影響因子(IF)】3.21

出版期刊《Experimental Cell Research》

【產(chǎn)品原文引用】

The tissues were fixed with 4% paraformaldehyde for 24 h, hydrated with 80%, 90%, 100% ethanol and n-butanol, embedded with paraffin, and cut into 5-μm-thick sections. After being heated at 60°C for 1 h, the sections were dewaxed with xylene, dehydrated with gradient ethanol (80%, 90%, 100%) and immersed in 3% H2O2 for 10 min. Antigen retrieval was performed, and the sections were then blocked with 100 μL 5% bovine serum albumin at 37°C for 30 min. Subsequently, the sections were incubated with 100 μL primary rabbit antibodies against SIX4 (1: 2000, ab176713), VEGF (1: 250, ab32152), and CD31 (1: 50, ab28364) overnight at 4°C followed by incubation with a secondary biotin-labeled goat anti-rabbit antibody (HY90046, Shanghai HengYuan Biological Co., Ltd., Shanghai, China) at 37°C for 30 min. Finally, the sections were stained with DAB chromogen (Beijing Bioss Biotechnology Co., Ltd., Beijing, China), and the cell nucleus was stained with hematoxylin for 5 min. The positive cells were stained in brown-yellow. Sections were visualized by Image-Proplus image analysis software (Media Cybernetics, Silver Springs, MD, USA). The target protein expression was evaluated by the proportions of positive cells in total cells detected from 5 randomly selected fields of view on every slide (200-fold magnification). Proportion scores were assigned as follows: positive tumor cells/total tumor cells > 10% positive (+), positive cells < 10% negative (-). 

完整版PDF文獻(xiàn)請咨詢在線客服或者聯(lián)系我司業(yè)務(wù)員!

更多公司福利請關(guān)注“恒遠(yuǎn)生物”VX公眾號!

聯(lián)


主站蜘蛛池模板: 精品无码国产日韩制服丝袜| 女人与公拘交酡全过程| 国产精品久久精品第一页| 野外少妇愉情中文字幕| 伊人久久大香线蕉综合影院首页| 久久性爱视频| 日本精品久久久久中文字幕| 亚洲最大无码中文字幕| 亚洲av无码一区二区三区观看| 无码国产精品一区二区免费式直播| 中国女人内射6xxxxx| 装睡被陌生人摸出水好爽 | 国产精品无码成人午夜电影 | 午夜免费无码福利视频| 大学生被内谢粉嫩无套| 久久亚洲av无码精品色午夜麻| 国产内射xxxxx在线| 国产精品污www在线观看 | 久久精品中文字幕有码| 国产剧情av麻豆香蕉精品| 荡女精品导航| 免费毛片在线看片免费丝瓜视频| 国内精品久久久人妻中文字幕| 欧洲熟妇乱xxxxx大屁股7 | 老司机亚洲精品影院| 日韩av高清在线观看| 国产午夜精品久久久久免费视| 波多野结av衣东京热无码专区| 亚洲а∨天堂久久精品2021| 我爱我色成人网| 蜜臀av一区二区| 国产艳妇av在线| 与子敌伦刺激对白播放的优点| 高潮爽死抽搐白浆gif视频| 日本熟妇厨房xxxxx乱| 亚洲精品无码av人在线播放| 老色鬼在线精品视频在线观看| 亚洲午夜无码久久久久蜜臀av| 美女裸体a级毛片| 成全动漫视频在线观看免费高清| 国产成人av无码专区亚洲av|